Embryo paraffin tissue sections by immunohistochemistry and Methods in the Problems of Improvement.docVIP

 PAGE \* MERGEFORMAT 11 Embryo paraffin tissue sections by immunohistochemistry and Methods in the Problems of Improvement On: Zheng Min Li Li Chi Mao Jianwen Wang Wei Wang Li Jing Zhang [Abstract] Objective To summarize the chicken embryo tissue immunohistochemical staining of operating experience. Immunohistochemical methods using routine methods at different developmental stages of embryo paraffin tissue sections for immunohistochemical staining. Results Immunohistochemistry was used to detect the early chick embryo Developmental expression of the relevant antibodies accurate positioning, a clear background. Conclusion Note immunohistochemistry chicken operation points, helping organizations improve the embryo quality of immunohistochemical staining. [Keywords:] chick; HE staining; immunohistochemistry Chick embryo material because of its low prices, the economy has important practical and experimental value, is widely used in oncology, medicine and other research in the field [1]. The immunohistochemical method is a common pathology technology, providing research and teaching Intuitive morphological support [2]. but because the market for less specific antibodies of chicken, chicken doing the rats commonly used in immunohistochemistry, rabbit or human antibodies and other easily accessible market, so often some Buffet Coloring the opposite sex and other issues. I embryo in the different periods of normal paraffin tissue sections for HE staining and immunohistochemical staining, we found some common problems embryo tissue, these issues were discussed and put forward improved method. 1 Materials and methods 1.1 Materials Eggs from the laboratory species of South China Agricultural University farms. Chick hatching 3 to 8 days, take 5 to 10 a day. The contents into crystalline chicken dish, carefully remove the embryo, with normal saline. The whole Embryos immersed in 4% () paraformaldehyde 4 ~ 24 h. paraffin-embedded, 5 m / chip paraffin s