Enterobacter cloacae plasmid-mediated quinolone resistance mechanisms of drug.docVIP

 PAGE \* MERGEFORMAT 9 Enterobacter cloacae plasmid-mediated quinolone resistance mechanisms of drug Author: Wu Jian Lu Deng Chuang Hung-wen, ZHANG Kou-xing 【Abstract】 Objective To understand the Shenzhen area Enterobacter cloacae in the prevalence of qnrA genes, gene targeting and its mediated quinolone resistance mechanisms generated. Methods in clinical isolates of Enterobacter cloacae 45, using PCR method combined with screening for qnrA gene sequencing technology, large plasmid extraction, Southern hybridization, and plasmid conjugal transfer experiments positioning, agar dilution method for drug susceptibility detection. Results 45 Enterobacter cloacae, the seven-PCR and sequencing confirmed qnrA gene. Seven strains of bacteria carried by the 6 plasmid have been successfully extracted and carried out Southern hybridization, qnrA gene located in 80 ~ 200kb size of the natural low copy number plasmid. 4 bacteria successful conjugal transfer test, so that zygote ciprofloxacin MIC values increased 32 ~ 64-fold. Conclusion plasmid-mediated quinolone resistance gene qnrA in Enterobacter cloacae in the Shenzhen area has a high prevalence may be the cause of the quinolone antibacterial agents Enterobacter cloacae acquired resistance to important reasons. Keywords: qnrA gene Enterobacter cloacae quinolone resistance plasmid Plasmid  mediated quinolone resistance in clinical isolates ABSTRACT Objective To investigate the prevalence and location of qnrA gene in clinical isolates of Enterobacter cloacae and its function in the development of quinolone resistance. Method Forty  five Enterobacter cloacae were screened for the qnrA gene by PCR and then conformed with direct sequencing. Large plasmid extraction and Southern hybridization were done to determine the location of qnrA. Conjugation experiments were done to determine the transferability and its effects in the development of quinolone resistance. Results qnrA were detected in 7