Epimedium total flavonoids extraction technology and content distribution.docVIP

 PAGE \* MERGEFORMAT 17 Epimedium total flavonoids extraction technology and content distribution Author: Liu Min, Zhou F, Huang Shao-wei, CHI Ru-an, Wu Yuan-yan, WANG Cun-wen, giant mix personal, Guo Jia, Huang Qi-Mao 【Key Words】 epimedium;,, extraction;,, total flavonoids;,, orthogonal design Abstract: Objective To study the extraction process of total flavonoids of Epimedium and the origin of different plants of Epimedium total flavonoids content in various parts of the distribution. Methods of ethanol and water reflux extract epimedium flavonoids; select Enshi, Hubei and Fujian Sanming epimedium as raw material, making single-factor experiment, and then to extract the rate of Epimedium Flavonoids the indexes for orthogonal experimental design; use UV spectrophotometry epimedium total flavonoids. The results with ethanol and water as extraction agent extraction rates were 70.5%, 31.1%; Enshi produced by Arrow with the Sanming Epimedium Epimedium total flavonoids of its contents were as follows: leaves 3.24%, 1.02%; stem 0.91% , 0.14%; root 2.47%, 0.61%. Conclusion Enshi, Hubei Province produced by Fujian Sanming than the Arrow Epimedium Epimedium sagittatum produced by high levels of total flavonoids; ethanol as extraction agent was significantly higher than the extraction of water, the impact effect of epimedium flavonoids extract the main sub-factors: the concentration of solvents “return time” extract the number of “liquid-solid ratio. Optimization of Epimedium flavonoids extraction technology as follows: liquid to solid ratio of 12:1, with 60% ethanol extract 3 times, 1.5 h / times, optimizing the process of total flavonoids extracted rate of 77.5%. Keywords: Epimedium; extraction; total flavonoids; orthogonal design The Extraction Technology for Total Flavone and its Content Distribution in Herba Epimedium Abstract: ObjectiveTo study an extraction Technology for total flavone and to determine the content distribution in