EPO on the expression of iron regulatory protein of Hepcidin.docVIP

 PAGE \* MERGEFORMAT 9 EPO on the expression of iron regulatory protein of Hepcidin Authors: SUN Xuefeng, Zhou Bin, Zhao Yongqiang Abstract This study investigated the erythropoietin (EPO) in normal mice, mice with acute inflammation and chronic anemia (ACD) mouse model of hepcidin mRNA expression. Pairs of mice subcutaneously through the inter-scapular fat pad injection of turpentine method of building model and the ACD model of acute inflammation. Normal mice, mice and ACD period of acute inflammation in mice after intraperitoneal injection of EPO using reverse transcription-polymerase chain reaction (RT-PCR) semi-quantitative detection of liver hepcidin mRNA expression levels. The results showed that chronic inflammation in the liver of mice was not significantly higher hepcidin mRNA expression, but the single injection of turpentine can make mouse liver hepcidin mRNA levels of a transient significantly increased. Intraperitoneal injection of EPO can inhibit the normal mice, ACD in mice with acute inflammation in mouse liver hepcidin mRNA expression. ACD in mice after injection of EPO levels and hemoglobin levels of liver hepcidin mRNA expression in a linear negative correlation. Conclusion: The repeated subcutaneous injection of turpentine ACD model can be established. ACD Early in the pathogenesis of liver hepcidin mRNA increased, after which the return to normal. EPO in normal conditions, acute inflammatory conditions and chronic inflammatory conditions, can inhibit the expression of hepcidin mRNA. Keywords: chronic anemia; hepcidin; erythropoietin; iron metabolism An Iron Regulator Hepcidin Is Affected by EPO Abstract To investigate the effect of EPO on hepcidin mRNA expression in normal mice, acute inflammatory mice and ACD mice, the acute phase model and ACD model of mouse was produced by subcutaneous injection of turpentine oil. Hepatic hepcidin mRNA expression levels of normal mice, acute inflammatory mice and ACD mice