Escherichia coli multidrug resistance integrons and the relationship between class.docVIP

 PAGE \* MERGEFORMAT 10 Escherichia coli multidrug resistance integrons and the relationship between class Of: Chen Wan-flower, Wu Yan An, Hu Xin Lan, Wu Changsheng, Li Ning [Abstract] Objective monitoring of clinical isolates of Escherichia coli resistance in Escherichia coli class to understand the prevalence of integrons and molecular features. Methods 114 strains of clinical isolates of Escherichia coli bacteria by automated analysis system to identify and Detection of drug resistance, application of plasmid DNA, PCR amplification of integron class on the PCR products were restriction analysis and DNA sequencing. will result in the GenBank sequence search to identify the variable region integron gene cassette arrangement of the type and . Results bacterial resistance ratesgt; 50%, and most of the multi-drug resistant (withstand more than 3 kinds of antibiotics.) plasmid in 58 strains of bacteria detected on DNA integron sequences, the size of about 600 ~ 3 500 bp, each containing 1 to 3 integron. integron gene cassettes of the most common is dfrl7 (trimethoprim resistance gene) and aadA5 (streptomycin, spectinomycin resistance gene), the most important gene cassette arranged dfrl7-aadA5. the vast majority of trimethoprim resistance gene carrying strains of cotrimoxazole resistance box, most carrying streptomycin, spectinomycin resistance gene cassette is not sensitive to the strains of streptomycin . Conclusion The clinical isolates of Escherichia coli resistant to strong and widespread class integron. strains carrying gene cassettes and the resistance phenotype of a good relationship between the gene box mediates bacterial resistance. bacterium multi-drug resistance rate of class integron positive rate related. [Keywords:] Escherichia coli O157; drug resistance; integrons; Genes, Bacterial; antibacterial drugs Escherichia coli is the most common human infection caused by Enterobacteriaceae [1], the drug situation is very grim. Bacterial