Escherichia coli producing PER 1-type extended spectrum β lactamases of.docVIP

 PAGE \* MERGEFORMAT 21 Escherichia coli producing PER 1-type extended spectrum β lactamases of 【Abstract】 Objective To detect Escherichia coli producing PER  1-type extended spectrum β  lactamases and drug resistance characteristics of the incidence. Methods The double disk synergy confirmatory test for ultra-extended-spectrum β  lactamases (ESBLs) phenotype testing phenotypic-positive strains by polymerase chain reaction (PCR) and PCR product sequencing method to determine the ultra-broad-spectrum β  lactamase genotypes. The results of 167 E. coli has 50 producing ESBLs, 29.9% (50/167); six middle PER  1-type ESBLs, accounting for 3.6% (6 / 167). 6 producing PER  1-type ESBLs are Escherichia coli to ceftazidime and cefotaxime resistant, five pairs of cefoxitin-resistant, four pairs of amikacin, all strains sensitive to imipenem. Conclusion Escherichia coli was detected in middle PER  1-type extended spectrum β -lactamase, and its resistance and multiple drug-resistant serious and should be attention. Keywords: Escherichia coli PER  1 β -lactamase-resistant genotype Study on PER  1 type extended  spectrum β  lactamase produced ABSTRACT Objective To investigate the prevalence of PER  1 type extended spectrum β  lactamases (ESBLs) and its antimicrobial  resistance profile in Escherichia coli. Methods One hundred and sixty  seven strains of Escherichia coli were tested. All of them were isolated from all kinds of clinical specimens from four hospitals of Nanchang city from August 2002 to June 2003. Antimicrobial susceptibility tests (AST) were determined by Kirby  Bauer disk diffusion test. Double  disk test and three  dimension extraction test were used to determine ESBLs. blaPER  1 genes were amplified by PCR. The products of PCR were sequenced to identify the β  lactamase genotype. Results The positive rates of ESBLs in Escherichia coli were 29.9% (50/167). Six isolates carried blaPER  1 gene. The blaPER  1 g