Eucalyptus leaves youmanii Determination of total flavonoids and quality of Identification of.docVIP

 PAGE \* MERGEFORMAT 23 Eucalyptus leaves youmanii Determination of total flavonoids and quality of Identification of 【Abstract】 Objective To study the youmanii eucalyptus leaves Determination of total flavonoids and quality of identification methods. Method with UV spectrophotometry, colorimetry aluminum chloride, aluminum nitrate colorimetric Sichuan youmanii Introduced Eucalyptus leaves flavonoids were determined and high performance liquid chromatography and the results were compared. Results Introduced youmanii methanol extract of leaves of Eucalyptus existence of A259/A364 ≈ 1.71 and A259/A364 ≈ 2.34 of the two kinds of quality of different plants by liquid chromatography analysis of their content and chemical composition of rutin significantly different. A259/A364 ≈ 1.71 of the four kinds of plants were measured result does not differ greatly, while the A259/A364 ≈ 2.34 aluminum nitrate plants were higher than the results of colorimetric determination of high-performance liquid chromatography and aluminum chloride, France 46.4%, 39.9%. Conclusion A259/A364 ≈ 2.34 plants may contain aluminum nitrate assay component interference. 3 aluminum chloride colorimetric method youmanii eucalyptus leaves flavonoids than UV and aluminum nitrate method; using hot water extraction, ultraviolet spectrophotometry and high performance liquid chromatography can easily be two types of plants leaf identification. Keywords: youmanii eucalyptus leaves; flavonoids; determination method; the quality of identification Study on Determination Methods of Total Flavonoids and Quality Identification about Eucalyptus youmanii Leaves Abstract: ObjectiveTo study the determination methods of total flavonoids and quality identification about Eucalyptus youmanii leaves.MethodsThe content of total flavonoids in leaves of Eucalyptus youmanii which was induced to Sichuan was detected by UV spectrophotometric method, AlCl3 colorimetric method, Al (NO3) 3 colorimetri