Evaluation of colloidal gold with the ELISA test card detection of hepatitis B virus serum markers in the performance of two pairs of semi.docVIP

 PAGE \* MERGEFORMAT 9 Evaluation of colloidal gold with the ELISA test card detection of hepatitis B virus serum markers in the performance of two pairs of semi Of: Chen Hongan, since the starting gate, Wang Ying, Jiang Wenjuan [Abstract] Objective enzyme-linked immunosorbent assay (ELISA) Evaluation of colloidal gold detection of hepatitis B test card two pairs of half performance. Methods ELISA detection of hepatitis B surface antigen (HbsAg) negative in 100 cases, 200 cases positive with colloidal gold test card and ELISA, respectively, two pairs of semi-contrast detection of hepatitis B results and the detection time. Results of colloidal gold test card testing a single sample time 25min, and the ELISA for 1h.100 patients with HbsAg-negative specimens were detected only 2 cases HbcAb ELISA test positive for the gold Card tested negative, the other cases the results were the same .200 HbsAg positive samples, colloidal gold assay test card of 6 cases HbsAg negative, in line with rate 97.0%; HBeAb 9 cases tested negative, in line with rates of 92.9%; 7 cases HbcAb were negative, compliance rate of 96.5%. Conclusion colloidal gold test card, compared with the ELISA does not require special equipment, easy observation and rapid detection of a single specimen, simple, high specificity, to meet emergency needs. [Keywords:] two pairs of semi-hepatitis B; colloidal gold test card; ELISA Abstract Objective To evaluate the performance of colloidal gold card in detecting HBV with ELISA.Methods Colloidal gold card and ELISA were applied respectively to 100 cases with negative HbsAg and 200 cases with positive HbsAg detected out by ELISA in detecting HBV, and analysis and evaluation were made.Result The detecting time for single sample was 25min by colloidal gold card and 1h by ELISA; out of the 100 cases with negative HbsAg, ELISA detected out 2 cases with negative HbcAb while colloidal gold card detected out all cases with negative HbcAb, except this, the