Estradiol#39;s immune colloidal gold test strip assay.docVIP

 PAGE \* MERGEFORMAT 15 Estradiol#39;s immune colloidal gold test strip assay 【Key Words】 Estradiol Estradiol (17β-Estradiol) is an important component of natural estrogen in order to prevent儿童性早熟issues such as food and poultry products in particular, estradiol testing can not be ignored. Colloidal gold immunochromatography assay (GICA) in the detection of small molecule organic compounds the increasing attention. The basic principle is to stabilize the colloidal gold particles adsorbed protein, while the protein had no significant change in biological activity, so it can replace the enzyme labeled antibody 〔1,2〕. In this study, using monoclonal antibodies tagged with estradiol colloidal gold particles and the corresponding fixed on the cellulose nitrate membrane combination of estradiol conjugates, fixed with estradiol conjugate detection of lines at the show the color clear 〔3,4〕. This method is high sensitivity, specificity, simple and rapid, low cost, easy interpretation of results, etc., applicable to initial screening of samples. 1 Materials and methods 11 reagents and instruments HAuCl (Shanghai Chemical Reagent Factory); bovine serum albumin, egg albumin, rabbit anti-mouse polyclonal antibody (Tianjin Union Star biotech companies); estradiol standard, estradiol monoclonal antibody, 17β - Estradiol-6-one6-(o-carboxymethvyoxime) U.S. Sigma Company); other reagents were of analytical grade. Cellulose nitrate membrane, glass fiber membranes (U.S. Millipore Corporation); Beckman Du530 spectrophotometer (Germany Beckman Corporation); low-temperature high-speed centrifuge (Military Academy of Medical Sciences); Point Film Machine (United States Bio-Dot Corporation). 12 Methods 121 Preparation of colloidal gold with three gold acid chloride dissolved in distilled water to final concentration of 01g / L. First conduct a boiling water bath until boiling chloride Gold acid solution, each 100ml and 1% sodium citrate 25ml, and