Escherichia coli O157- H7 Detection Method.docVIP

 PAGE \* MERGEFORMAT 17 Escherichia coli O157: H7 Detection Method 【Abstract】 Objective Escherichia coli O157: H7 detection to explore, to reduce the daily workload, cost savings, increase the positive detection rate. Methods PCR technology and the Combination of immunomagnetic beads, samples for PCR detection of the first positive samples longer immune beads were separated and identified. The results from the 245 pig manure, cow dung samples combination of immunomagnetic bead and PCR method, the positive detection rate of 8.98%, higher than the direct application of MACS positive detection rate of 6.12%. Conclusion This method for Escherichia coli O157: H7 detection provides a new idea, saving manpower and material resources to improve the working efficiency and the positive detection rate. Keywords: Immunomagnetic method; Escherichia coli O157: H7; PCR technique Study on method for detecting E.coli O157: H7 【Abstract】 Objective To discuss the detection method for E.coli O157: H7.We can economize cost, release the work, enhance the positive rate.Methods Combined the polymerase chain reaction technique with immunomagnetic sand method, we could detect these samples with PCR at first, and then collected and detected the positive species by using immunomagnetic sand method.Results We have detected 245 species of dung by using this operational procedure. result proved that the positive rate can be up to 8.98% above the rate of 6.12% by using immunomagnetic sand method only.Conclusion It is a new idea for us to release the work and enhance the positive rate by using this operational procedure for detect the E.coli O157: H7. 【Keywords:】 immunomagnetic sand method; E.coli O157: H7; polymerase chain reaction Since 1982, the first in the United States by E. coli O157: H7 cause hemorrhagic colitis outbreaks since the bacteria in the world have had several foodborne outbreaks. Occurred in Japan in 1996