Everted rat intestine model for study of absorption mechanism of puerarin.docVIP

 PAGE \* MERGEFORMAT 8 Everted rat intestine model for study of absorption mechanism of puerarin Author: Choi Seung-Miao, Zhao Chunshun, HE Zhong-gui [Abstract] Objective To establish an accurate, sensitive and reliable high performance liquid chromatography puerarin (HPLC) determination method to study the existence of sub-puerarin intestines to absorb the difference and look at intestinal epithelial tissue on the p-glycoprotein (P-glycoprotein) puerarin absorption. Method in different intestines (jejunum, ileum and colon) of the intestinal eversion model by adding puerarin Krebs solution, sampled at different time points and to measure drug concentration in capsule compare the permeability of puerarin in different intestines differences in ability, while Observation of p-glycoprotein inhibitors, digoxin, and verapamil on the absorption of puerarin. The results of puerarin in the jejunum, ileum and colon to absorb all have their own inhibitory concentration. With the p-glycoprotein inhibitors, digoxin, and verapamil increased the concentration of puerarin enhanced through the intestinal mucosa, when the concentration reached 50 mg * L-1, and 200 mg * L-1, the This role has a certain degree of saturation. Conclusion puerarin absorption in the intestines may be the existence of active transport processes, puerarin is a p-glycoprotein substrate. [Keywords:] puerarin; intestinal eversion model; absorption; verapamil; digoxin) Abstract: ObjectiveTo develop an accurate, sensitive and reliable HPLC method for puerarin, and to study the absorption characteristics of puerarin in different intestinal sections (jejunum, ileum and colon) and the influence of p-glycoprotein on the absorption rate.MethodsRat everted gut sac was used. Sac containing drug free Krebs solution was immersed into the Krebs solution containing puerarin. The solution inside the sac was collected and drug concentration was determined by HPLC method. Digoxin and verapamil were used to study the