Extracts of black sesame B16 melanoma cells to promote melanin synthesis and its mechanism.docVIP

 PAGE \* MERGEFORMAT 16 Extracts of black sesame B16 melanoma cells to promote melanin synthesis and its mechanism Of: Jiang Ze group, Xu Jimin, Wu Qiong, Guang-Yuan [Abstract] Objective To study the black sesame seed extract on tyrosinase activity in B16 melanoma cells, melanin synthesis and the related gene and protein expression to explore the black sesame seeds for the possible mechanism of melanin synthesis. Methods 3 concentration (0.5,1,2 mg / ml) of water extracts of black sesame effect on B16 melanoma cells 72 h, observed on the proliferation of melanocytes, tyrosinase activity and melanin content. immunoblotting method and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) were detected 48 h after drug tyrosinase (Tyrosinase), small eyes associated transcription factor (microphthalmia associated transcription factor, MITF), tyrosinase related protease 1 (TRPl) and tyrosinase-related protein 2 (TRP2) protein and mRNA expression levels. Results 3 concentrations of aqueous extract of black sesame seeds may be slightly promote the proliferation of B16 cells (Pgt; 0.05); tested concentration range B16 cells could significantly promote the melanin synthesis and tyrosinase activity increased (P lt;0.05), and a concentration dependent manner to promote tyrosinase and MITF protein and mRNA expression (P lt;0.05), but TRP-1 , TRP-2 expression had little effect (Pgt; 0.05). Conclusion The aqueous extract of black sesame in vitro B16 melanoma cells can directly stimulate the synthesis of melanin, the change may be through the promotion of tyrosinase and MITF gene transcription and protein to complete the role. [Keywords:] aqueous extract of black sesame seeds; tyrosinase (Tyrosinase); black pigment; small eye-related transcription factor (MITF); tyrosinase related protein 1 (TRPl); tyrosinase related protein 2 (TRP2 ) Abstract: ObjectiveTo study the effect of water extract from Semen Sesami Nigrum (WES) on cell viability, tyros