Eye LUOTONG granule quality control standards for laboratory research.docVIP

 PAGE \* MERGEFORMAT 14 Eye LUOTONG granule quality control standards for laboratory research Author: Jiang Ning Fu Ying Hua Hua Xu Hongxiang [Abstract] [Objective] eye LUOTONG granules qualitative and quantitative indicators of quality control. [Methods] G thin-layer chromatography on silica gel granules in the two main components, and Tri-chuanxiong qualitative identification; by RP  HPLC method for determination of puerarin, using Kromasil 100  5C18 (4.6mm * 250mm, 5μ m) for the column, methanol - water (30:70) as mobile phase, detection wavelength of 250nm. [Results] puerarin injection volume of 0.06 ~ 0.65μ g and peak area within the framework of a good linear relationship, correlation coefficient r = 0.9999, day, day precision RSD were 0.85% and 0.48% (n = 5 ). High, medium and low concentrations of 3 The average recovery (n = 3) were 101.2%, 101.1%, 101.4%, RSD were 1.1%, 0.94%, 0.41%. [Conclusion] The silica gel G thin-layer chromatography RP-HPLC qualitative method and quantitative method is reliable and accurate, reproducible, can be used as quality control standards of this preparation. [Keywords:] RP-HPLC; thin-layer chromatography; puerarin; angelica; Tri; eye LUOTONG granules Abstract: [Objective] To observe the indexes of quality control of nature and quantity of Yanluotong Infusion. [Method] Make authentication to the 2 main components of Ligusticum wallichii and rhizoma sparganii on their nature with Silica gel G thin  layer chromatography; make content measurement to puerarin with RP  HPLC, take Kromasil 100  5 C18 (4.6mm * 250mm, 5μ m) as chromatogram column, methanol  water (30:70) as flow phase; the measured wavelength is 250nm. [Result] The puerarin sampling in the range 0.06 ~ 0.65μ g is in good linear relationship with the summit area, the relative coefficient r = 0.9999, the intraday and days nicety RSD are respectively 0.85% and 0.48% (n = 5); the average sample reclaim rates of high , middle and low concentrations (n =