Fetal liver cell line immortalized Construction and biological characteristics of.docVIP

 PAGE \* MERGEFORMAT 6 Fetal liver cell line immortalized Construction and biological characteristics of Author: Li Yu, snow sails, Wang Jun, Xu Zhe, Zhang Yan [Keywords:] immortalization; liver cells; cell line; biological characteristics; SV40; liposomes Establishment and biological characterization of an immortalized human fetal hepatocyte line [Abstract] AIM: To establish an immortalized human fetal hepatocyte line and to study its biological characteristics and functions. METHODS: Human fetal hepatocytes were transfected with pcDNA3.1 recombined vector containing the Simian Virus 40 large T antigen. Characteristics of the immortalized fetal hepatocyte line were evaluated by morphologic and functional detection. RESULTS: One of the hepatocyte clones displaying highly differentiated in liver function and immortalized characteristics had been screened after 40 day selection of 700-300 mg / L G418. The immortalized hepatocyte line maintained the most morphologic characteristics of primary hepatocyte. The cell cloneforming rat was 31.2%. The immortalized human fetal hepatocytes had a normal karyotype and were not able to grow in soft agar culture. It had the ability of composing albumin and the immunohistochemical test showed that the SV40T gene had been integrated into the transformed cell genome. CONCLUSION: The immortalized human fetal hepatocyte line has the similar most morphologic characteristics and biological function to primary hepatocytes, and it would become ideal cell material on the study of bioartificial liver and hepatocyte transplantation. [Keywords] immortalized; hepatocytes; cell line; characterization; SV40; liposomes [Abstract] Objective: To construct immortalized liver cell line and its biological characteristics and some of the features studied. Methods: SV40T genes and eukaryotic expression vector pcDNA3.1 by Lipofectamine to the in vitro isolation and culture of human embryonic liver cells to immortalization, further identification