Fetal mouse cerebral cortex neural stem cells from cultured differentiation and identification of.docVIP

 PAGE \* MERGEFORMAT 13 Fetal mouse cerebral cortex neural stem cells from cultured differentiation and identification of Author: HU Xiao-xia, ZHU Zhu-Xia, Chen Gang, Tan Jie [Abstract] Objective: To establish fetal mouse cortical neural stem cells isolated and cultured, differentiation and identification of ways to explore the basic biology of neural stem cell characteristics. Methods: The pregnant 14 d fetal mouse cerebral cortex, wind and percussion mechanical separation of straw made into single cell suspension, using serum-free culture and fetal bovine serum-induced differentiation, immunofluorescence Identification of neural stem cells and their differentiated daughter cells. Results: isolated from fetal rat cerebral cortex cells were nestin (Nestin) immunoreactivity and can rumored in vivo cultured cells after serum-induced differentiation can be expressed neurons, glial cells, microtubule-associated protein-specific antigen 2 and glial fibrillary acidic protein, induced to differentiate into neurons can express specific markers of synapse Synatophymin. Conclusion: The isolation and culture of cells can express neural stem cell-specific markers Nestin, and has a strong proliferative capacity and multilineage differentiation potential, belonging to the central nervous system neural stem cells. [Keywords:] stem cell differentiation in cerebral cortex cells in cultured cells, embryonic Sprague Dawley rats [Abstract] Objective: To establish a method for isolation, cultivation, differentiation induction, and identification of neural stem cells (NSCs) from embryonic rat in vitro, and explore the basic biological features of these cells. Methods: The mesencephalon of rat with conceptus age of 13 days was taken out and dissociated into single cell suspension mechanically, and then cultured in serum  free medium. Fetal bovine serum was used to induce cell differentiation. The isolated cells and their progeny were observed by using immunofluorescence techniq