Flow injection chemiluminescence study of flavonoid substances such as the role of superoxide radical.docVIP

 PAGE \* MERGEFORMAT 9 Flow injection chemiluminescence study of flavonoid substances such as the role of superoxide radical Author: Fang Zhao Bian Li Du Zhijian Zhang Ling [Abstract] Objective To establish a new method to study chemiluminescence flavonoids and other substances on superoxide radical scavenging effect. Methods under alkaline conditions of pyrogallol autoxidation as the superoxide anion generation system to reduce the chemiluminescence as the detection of activity indicators. The results of Chrysanthemum, Portulaca oleracea, quercetin and rutin were shown pairs of superoxide anion radical scavenging effect. Conclusion The method is simple, rapid, and can be used to study antioxidant activity of Chinese herbal medicine. [Keywords:] Chemiluminescence flavonoids superoxide anion radical Abstract: ObjectiveTo determine the scavenging effect of flavonoids ect. on superoxide radicals. MethodsReduction of chemiluminescence worked as an index with pyrogallic acid self-oxide in alkaline medium. ResultsChrysanthemum, purslane herb, quercetin, rutin showed scavenging effect on superoxide radicals. ConclusionThe method is simple and quick. It can be used for determination of antioxidant activity from Chinese herbal medicines. Keywords:: Chemiluminescence; Flavonoids; Superoxide Radicals Life activities of aerobic metabolism can continue to produce a variety of free radicals, superoxide anion radical O-2 * is one of the three kinds of representative radicals (superoxide anion radical O-2 * , hydroxyl radical OH * , Ester radicals RCOO * ) one. [1] of excess superoxide anion radical O-2 * on biological cells have great destructive effect is the cause of human aging and lead to cancer, coronary heart disease, arteriosclerosis and other diseases, one of the incentives. Vivo clearance of excess free radicals, seeking a clear anti-oxidant activity of oxygen free radicals in medicine and biology has become an important research topic. The free radical det