Fm04 curcumin derivatives inhibit proliferation of K562 cells and its impact on P210bcr-abl signal pathway.docVIP

 PAGE \* MERGEFORMAT 5 Fm04 curcumin derivatives inhibit proliferation of K562 cells and its impact on P210bcr/abl signal pathway Authors: Li Na, Wu Lixian, WEN Cai-xia, HUANG Xiu-wang, Xu Jianhua [Abstract] Objective To study curcumin derivatives Fm04 on K562 cell proliferation and the right P210bcr/abl signal pathway. Methods MTT assay Fm04 curcumin derivatives on K562 cell proliferation; application of Western blot to detect P210bcr/abl and its downstream pathway protein expression changes and changes in mitochondrial cytochrome C; colorimetric assay Caspase  3, Caspase  9 activation. Results Fm04 of K562 cells in vitro dose-effect, time-effect relationship, 4 μ mol / L Fm04 role of 24 h, inhibition rate of 88.4%, half inhibitory concentration of 1.45 μ mol / L, intensity of curcumin is about 7 times; Western blot showed that Fm04 P210bcr/abl significantly reduced and its downstream signaling pathway proteins, reducing mitochondria cytochrome C levels; Fm04 treatment group Caspase  3, Caspase  9 concentration increased. Conclusion Fm04 significantly inhibited the proliferation of K562 cells and to reduce the protein content of P210 and thus lower P210bcr/abl a variety of signaling molecules downstream of the expression by activating caspase activation and mitochondrial release of cytochrome C, and eventually induce apoptosis of K562 cells. [Keywords:] curcumin; apoptosis; leukemia, myeloid, chronic; fusion protein; bcr  abl; K562 cells; signal transduction ABSTRACT: Objective To study the apoptosis  inducing effects of the synthetical curcumin derivative Fm04 on chronic myelogenous leukemia (CML) cell. Methods MTT was used to determine the apoptotic effects of Fm04 on K562 cells. The signal protein molecules expressed in K562 cells was examined by Western Blotting. The spectrophotometer kit was used to detect the activity of Caspase  3 and Caspase  9. Results Exposure of K562 cells to Fm04 produced both concentration  and time  depe