Gel Permeation Chromatography Cleangas chromatography of organochlorine pesticides in traditional Chinese medicine residues.docVIP

 PAGE \* MERGEFORMAT 12 Gel Permeation Chromatography Cleangas chromatography of organochlorine pesticides in traditional Chinese medicine residues Author: Xia Pin-Hua Zhang Wen-Sheng Chen Ng Hing, when [Abstract] Objective To establish a gel permeation chromatography (GPC) clean - traditional Chinese medicines by capillary gas chromatography of organochlorine pesticide residues method. Method of gel permeation chromatography used a mobile phase of acetone - cyclohexane (1:4), the best flow rate was 5 ml / min, fraction collection of the best time is 15 ~ 26 min. The results of low, medium and high levels of 3 to add recoveries were 85.5% ~ 115.0%, 84.3% ~ 123.4%, 86.7% ~ 114.0%, relative standard deviation (RSD) of 0.1% ~ 9.3%, detection limit 0.008 ~ 0.05μ g/kg. Conclusion The method is simple, a good separation with high sensitivity and good reproducibility. [Keywords:] Gas Chromatography-Gel permeation chromatography of organochlorine pesticide residues in Chinese medicine Organochlorine pesticides (OCPs) are a class of highly efficient broad-spectrum insecticide, is widely used to kill agricultural pests, was once the most widely used pesticides States of a large class. Pesticides and chemical properties of such stable, fat-soluble strong residual valid long, easy to accumulate in the fat body, resulting in chronic poisoning, a serious threat to human health, Chinese herbal medicines and proprietary Chinese medicines of pesticide residues in recent years, the subject of growing attention. The establishment of pesticide residues in Chinese herbal medicine as soon as possible detection methods and the limited standards, drug safety guarantee that people will have a positive meaning. Chinese herbal medicines in the current determination of organochlorine pesticide residues in the major organic solvent extraction, concentrated sulfuric acid purification, gas chromatographic analysis. But the complex Chinese herbal medicinal ingredients, which are trace amo