Glucose in human liver cells angiopoietin-like protein 3 expression in.docVIP

 PAGE \* MERGEFORMAT 2 Glucose in human liver cells angiopoietin-like protein 3 expression in Authors: Li Qian, WANG Ji-hong, CHENG Mei, Wang Xin, Zeng Jian-Tao [Abstract] Objective: To observe the high concentration of glucose in human liver cell line (L02) and the cell model of insulin resistance (IR  L02) of the angiopoietin-like protein 3 (ANGPTL3) expression, and to explore ANGPTL3 and diabetes (DM) complicated with lipemia relationship between the DM at the cellular level analysis of complicated molecular mechanism of hyperlipidemia. Methods: L02 as the research object, and the establishment of models of insulin resistance (IR  L02). L02, and IR  L02 containing two sets of cells were 5.6,7.0,11.1,28.0 and 33.0 mmol / L glucose culture medium, 5.6 mmol / L group as the control group. by RT  PCR used to detect the mRNA expression of ANGPTL3 and used Western Blot to detect ANGPTL3 protein expression levels, Analysis ANGPTL3 with DM complicated by hyperlipidemia. Results: high concentration of glucose can promote IR  L02 and L02 two-cell ANGPTL3 of mRNA and protein expression and showed a definite dose-effect relationship between changes in the differences were statistically significant ( P