Green June outside the petroleum ether extract objects SGC-7901 gastric cancer cells apoptosis and its mechanism of.docVIP

 PAGE \* MERGEFORMAT 18 Green June outside the petroleum ether extract objects SGC-7901 gastric cancer cells apoptosis and its mechanism of Of: Zhang Zhiwei, REMEDIES [Abstract] Objective To study the petroleum ether extract of green June (petroleum ether extract of LYQ, LYQPE) on human gastric cancer SGC-7901 cell apoptosis and some of its mechanism of action. Methods with different concentrations of LYQPE role in SGC-7901 , the role of 48 h, flow cytometry detection of apoptosis and apoptosis-related protein p53 effects; RT-PCR detection of apoptosis related genes p53 mRNA and CD44 mRNA changes in adhesion gene; electron microscope ultrastructure. Results LYQPE vitro on SGC-7901, at 40 ~ 320 μg / ml for the time showed different degrees of apoptosis; in a dose-dependent inhibition of p53 protein, mRNA level and the level of CD44 mRNA expression; at 160μg/ml, the electron microscope the typical morphological changes of apoptosis. Conclusion LYQPE on SGC-7901 significantly induce apoptosis; LYQPE inhibition of SGC-7901 p53 protein, mRNA level and the level of CD44 mRNA expression may promote apoptosis of SGC-7901 mechanism . [Keywords:] June petroleum ether extracts of green; SGC-7901; flow cytometry; apoptosis; RT-PCR; electron microscope Gastric cancer is one of the most common tumors, and its death toll in China ranks first in malignant tumors [1]. Surgery for early gastric cancer has a good effect, but poor efficacy in advanced gastric cancer. So look for better efficacy, side effects small treatment is still the focus of cancer research. June Qing Xiao, Department of Acanthaceae plant aboveground dry cages is a kind of folk medicine in Guangxi. lt;lt;Herbal Supplementsgt;gt; records, it can be blood, cool blood, liver and wet diarrhea, swelling and pain, mainly for acute and chronic hepatitis, jaundice, etc. [2]. Chinese medicine theory g liver spleen, stomach and internal organs as the spleen, stomach and spleen relative to table, indicating t