Guinea pig hippocampal neural stem cells and biocompatibility of two different carrier materials.docVIP

 PAGE \* MERGEFORMAT 4 Guinea pig hippocampal neural stem cells and biocompatibility of two different carrier materials Author: Guobao Feng, Dong-Min, Guo Zhang-sheng [Keywords:] neural stem cells Biocompatibility between two different biomaterials and cultured hippocampusderived neural stem cells in vitro [Abstract] AIM: To study the biocompatibility between two different biomaterials (collagen protein sponge and glutin sponge) and cultured hippocampusderived neural stem cells in vitro and to identify whether the materials could be used as scaffold biomaterials in peripheral neural tissue engineering. METHODS: Hippocampusderived neural stem cells of guinea pigs were cultured and respectively combined with each of the two biomaterials in vitro. The number of cells was counted and the histological changes were observed with HE staining and scanning electron microscope after 7 d and the adhesion rates were detected. RESULTS : Hippocampusderived neural stem cells attached to both biomaterials and normally grew. The adhesion rates were 31.17% and 14.87% respectively. The total numbers of the cells in the experimental and control groups were similar and there was no statistical difference. CONCLUSION: Both biomaterials have a good biocompatibility with hippocampusderived neural stem cells of guinea pigs and can be used safely as scaffold materials in peripheral neural tissue engineering. [Keywords] neuronal stem cells; tissue engineering; biomaterials; biocompatibility; guinea pig [Abstract] Objective: To evaluate the guinea pig hippocampal neural stem cells and two kinds of different carrier materials (collagen sponge and gelatin sponge) for biocompatibility, to explore them as peripheral nerve tissue engineering feasibility of carrier material. Methods: The in vitro newborn dolphin Rat hippocampal neural stem cells, spread to the first three generations, the density of 1 * 1010 / L cells, respectively with two kinds of carrier material of the Commonwealth ou