Green tea extract and Table epigallocatechin gallate Study on the role of immune cells.docVIP

 PAGE \* MERGEFORMAT 11 Green tea extract and Table epigallocatechin gallate Study on the role of immune cells Authors: Zhang, Gui-Wen Yang, Zhang Fumiao, Zhao Jing, Cui Yingjie, Li-Guo [Abstract] Objective To study the green tea extract and table epigallocatechin gallate (EGCG) on the spleen cell proliferation and macrophage NO secretion. Methods MTT assay changes in spleen cell proliferation, using Griess reagent to detect changes in NO production. The results of green tea extract and EGCG can promote the proliferation of spleen cells and can be coordinated conA stimulate the proliferation of spleen cells; also able to inhibit LPS-activated RAW264.7 macrophages, NO production. Conclusion green tea extract and EGCG with immune regulation and reduce the beneficial effects of inflammation. [Keywords:] Green tea table epigallocatechin gallate immune cells Abstract: ObjectiveTo study the effect of green tea extract and Epigallocatechin gallate (EGCG) on spleen cell proliferation and macrophage NO production.MethodsMTT assay was used to detect spleen cell proliferation change and Griess reagent method was used to detect macrophage NO secretion. ResultsGreen tea extract and EGCG could promote spleen cell proliferation and be able to cooperate with conA to stimulate spleen cell proliferation.They could also inhibit LPS-activated macrophages RAW264.7 NO production.ConclusionGreen tea extract and EGCG have immunoregulative effect and can alleviate inflammatory reaction. Keywords:: Green tea; EGCG; Immune cells Modern research shows that green tea has anti-oxidation, anti-cancer and anti-cancer, lowering blood sugar, antibacterial and anti-virus and other biological activity [1 ~ 5]. There is a growing emphasis in health care today, a series of biological activity of green tea is no doubt also become a research hotspot. In this study, selection of Shandong Rizhao green tea as the material, with 70% ethanol extract of green tea extract obtained to study its effec