Gynostemma Optimization of extraction conditions of quercetin.docVIP

 PAGE \* MERGEFORMAT 9 Gynostemma Optimization of extraction conditions of quercetin [Abstract] Objective To optimize the extraction of quercetin in terms of Gynostemma. Methods Gynostemma of quercetin as an indicator, using liquid chromatography, using orthogonal experimental design L9 (34) optimized experimental conditions, comparative study of microwave-assisted extraction (MAE) and Soxhlet extraction and extraction at room temperature dipping Jiaogulan the rate of extraction of quercetin. The results identified the MAE Gynostemma extract the best conditions of quercetin as follows: particle size 160 ~ 250 μ m, moisture content 15%, power 100 W, extraction time was 10 min. Gynostemma Extract optimized MAE extraction yield of quercetin in the peso’s extraction method and the impregnation method at room temperature increased by about 46.2% and 63.3%, extraction time, Soxhlet extraction is only 1 / 24 times the impregnation method at room temperature 1 / 144-fold. Conclusion MAE to extract not only high, but also greatly shortened extraction time, is a suitable Gynostemma quercetin fast and efficient extraction method. [Keywords:] Jiaogulan quercetin orthogonal design of microwave-assisted extraction high-performance liquid chromatography Gynostemma Gynostemm apentaphyllum (Thunb.) Makino, GP, also known as Aescin gallbladder, five leaf ginseng, ginseng, etc. Aescin is Jiaogulan Cucurbitaceae species, the sprawling perennial herb. With a wide range of pharmacological effects, in the anti-aging, anti-fatigue, anti-radiation and the elimination of free radicals at the same time, but also to improve nervous system function, anti-ulcer, inhibit gallstone formation and regulation of endocrine activities [1], with the protection of heart, brain, blood vessels and liver, lower blood lipids, lowering cholesterol, lowering transaminase, regulation such as immunization and anti-mutagenic effects [2 ~ 4]. Therefore, the study of Gynostemma majority of scholars at home