H5N1 avian influenza vaccine for human use poisonous kinds of test and the establishment of toxic species pool.docVIP

 PAGE \* MERGEFORMAT 20 H5N1 avian influenza vaccine for human use poisonous kinds of test and the establishment of toxic species pool Author: Zhu Zhiyong, Ding Hang, Zhu Han-ping, LI Yan Jin, Chen Xue-Kui, Shen brothers, Zhang Tao, Pei-jiang, YAO Ping-ping, Xu, Weng-Qing, Gong Yue, Guo Zhijun, Su Bo, Sun Shubin, Liang Weifeng Keywords: bird flu vaccine; toxic species; test; toxic species pool Abstract: Objective To develop H5N1 avian flu vaccine for human use, according to “Chinese Pharmacopoeia” 3 “biological products production and test management protocols with the kinds of bacteria poisoning” in the request, the establishment of bird flu vaccine for the production of toxic species pool. Methods imported from abroad two H5N1 bird flu vaccine virus seed (R1194 and R1203), conduct a comprehensive inspection, the establishment of three seed bank, the former generation, the main generation and job generation for vaccine production. The results introduced in R1194 and R1203 2 Ge strains can Peidan proliferation and stability in a large number of passage; with ordinary influenza A1, A3, B for cross-hemagglutination inhibition test and one-way cross-immunodiffusion tests showed that in line with H5N1 avian influenza virus features; by the sequencing of the hemagglutinin, confirmed the introduction of two strains of H5N1 avian influenza virus; measured strain sequence with the original sequence comparison we can see that two strains have been to remove toxic gene, is a weak strain; determining 2 the original generation of the P2 strain, P4-based generation, P5 working on behalf of. Conclusion introduction of R1194 and R1203 toxic species by H5N1 avian influenza strain can be used for bird flu vaccine production. Keywords:: Avian influenza vaccine; toxic species; test; toxic species pool Study on avian influenza (H5N1) vaccine used in human body and establishment of virus seed bank Abstract: Objective To develop avian influenza (H5N