Guangdong region of avian influenza H5N1 hemagglutinin gene characteristics and evolution of.docVIP

 PAGE \* MERGEFORMAT 9 Guangdong region of avian influenza H5N1 hemagglutinin gene characteristics and evolution of Author: Huang, Ke Changwen, Zouli Rong, Li Hui, Chelsia Chan Keywords: human avian influenza; H5N1; strain; hemagglutinin (HA) gene; evolution Abstract: Objective of the Guangdong region of human avian influenza H5N1 strain (HA) gene sequence variation analysis, revealed strain HA gene mutation and evolution. Methods in Guangdong H5N1 strain of bird flu (A/GD/01/06) HA gene sequencing, and retrieval of people around the world H5N1 strain of avian influenza HA genes, using SPSS110 and DNAStar50 software to search H5N1 bird flu drug strain HA gene nucleotide sequences clustering, comparison and analysis; and combined with clinical data on the variation of speed of evolution of strains analyzed. The results from 1997 to 2006, 42 strains of HA gene sequences cluster into three categories; HA gene 89 amino acid replacement sites, accounting for 157% (89/568); from 2003 to 2006, H5N1, strain through amino acid 170 ~ 172 (NST / S) locus of the replacement, addition of a glycosylation site. Synonymous mutation in, HA gene Ks of 199 × 10-5 ~ 2.58 × 10-5, biological evolution linear regression equation Y = 48 × 10-9X 1.048 × 10-5; the same time, the strain of Ks are larger than the Ka , evolutionary testing Ks / Ka value is displayed HA gene evolutionary pressures mainly from the natural variation. 42 H5N1 strain can be divided into three evolutionary pathway, from 1997 to 1998 as a strain, strain in Southeast Asia from 2003 to 2005 as the first two channels, 2005 and 2006 strains in China for the first three channels. Strain HK-213-03 amino acid variation shows the evolution of the excessive nature of their variation, while the 2004 strain TL-L2004-04 amino acid point mutations is noteworthy. Conclusions from 2003 to 2006 human H5N1 strain of avian influenza HA antigen gene in 1997, a greater strain is different from human avian influenza H5N