Hai-feng vine extract on Na K-ATP enzyme activity of.docVIP

 PAGE \* MERGEFORMAT 9 Hai-feng vine extract on Na K-ATP enzyme activity of Author: Shen Fang, Yang Li Jiang, Lu Bin, Wang Debin 【Abstract】 Objective To confirm the study of traditional herbal medicine Yi - Hai-feng, whether the vine has a strong mind with the role of the active ingredient, and a preliminary understanding of the location and the presence of the active ingredient efficacy. Methods Myocardial Na, K-ATP enzyme activity assay used to detect whether the Hai-feng, vine extract substances with a strong heart. Results Hai-feng, vine stems and leaves contain inhibit Na, K-ATP enzyme activity of the substances; the inhibitory effect of stem extracts significantly better than other, and their cardiac activity was positively correlated with the dose; with the cardiac glycosides digoxin comparison of material , Hai-feng, cane water extract and digoxin on the myocardial Na, K-ATP inhibition of enzyme activity was no significant difference. Conclusion Hai-feng, Teng extract cardiac glycosides that have similar active ingredients exist, the active substance is mainly present in the stem, and stem water extracts containing composition and the strong heart of digoxin cardiac effects similar to. Keywords: Hai-feng, Teng Na K-ATP enzyme cardiac Abstract: ObjectiveTo study the effect of the extracts from Marsdenia officinalis Tsiang et PTLi on Na, K-ATPase activity and to affirm some cardiotonic materials in the plant.MethodsThe extracts were separated from stems, fruits and leaves of Marsdenia officinalis Na, K -- ATPase activity of SD rat heart sarcolemma was determined.ResultsThe extracts from stems and leaves of Marsdenia officinalis could inhibit the activity of Na, K-ATPase, and the extract from the stems had a stronger inhibition to the activity of the Na, K-ATPase than the others, and showed a dose-dependent manner. At the same time, the hydro-extraction from stems had no significant difference with Desacetyllanatoside in inhibitin