Guangxi total alkaloid extract a little red and antibacterial activity of.docVIP

 PAGE \* MERGEFORMAT 12 Guangxi total alkaloid extract a little red and antibacterial activity of Author: Zhou Jun-Sheng Li Yan Wu Ping WEI Yuan-yuan LIU Juan Ms Betsy [Abstract] Objective To investigate a little red in Guangxi separation and purification of total alkaloids and their antibacterial activity. Methods 95% ethanol was extracted in a little red medicinal alkaloid substances, the use of acidic water extraction and organic solvent extraction of its isolation and purification, while knowledge of chemical inspection method and thin-layer chromatography for qualitative identification. Study using filter paper a little red antibacterial activity of alkaloid substances. The results of a little red alkaloid concentration in the 600 ~ 800 mg / ml when Staphylococcus aureus, Escherichia coli and Bacillus subtilis moderately sensitive, with the drugs (alkaloids) concentration increases, the more obvious anti-bacterial effect. Findings make it clear that Red alkaloid substances tested strains have some antibacterial activity. [Keywords:] a little red antimicrobial activity of total alkaloid extraction and separation of Abstract: ObjectiveTo investigate the extraction and separation methods and antibacterial activities of the alkaloids in Emilia sonchifolia from Guangxi.MethodsThe alkaloids in Emilia sonchifolia were reflux extracted by 95% alcohol, and separated by acid water extraction and organic solvent extraction method, at the same time qualitative identification was performed by the chemical methods and the thin layer chromatography methods. To study the antibacterial activities of the alkaloids in Emilia sonchifolia by filter paper method. Results The result showed it was medium sensitive to Escherichia coli, Bacillus subtilis and Staphylococus aureus when the concentrations of the alkaloids were in the range of 600 ~ 800 mg / ml. The antibacterial function was more remarkable with the medicine concentrations increased. ConclusionThe alkaloids of Emi