High altitude plains and low-population genetic polymorphism related genes.docVIP

 PAGE \* MERGEFORMAT 14 High altitude plains and low-population genetic polymorphism related genes Author: Yu Moon-tong, WANG Xiao-qin, Qiu-Hong Chen, Shiming, Wu Tien-an [Abstract] Objective In this paper, genetic testing technologies, to understand the different groups of gene frequency, genotype frequency and characteristics of ACE gene and hypoxic regulation of gene (HIFG) relationship. Methods Polymerase chain reaction (PCR) gene amplification and polyacrylamide gel (PAG) shifter and gene sequencing technology and the plateau native Tibetan, Mongolian, Han and plains clan has lived in four groups of Han Ang converting enzyme gene (ACE) polymorphism. The results measured gene frequency of 0.6562 for the plateau Han, Tibetan 0.6377, 0.6505 and plains Han 0.7186 Mongolian plateau three groups, no significant difference between, respectively, compared with the plain the difference was significant (P amp;lt;0.001), genotype frequency of the plateau Han for I / I 14%, I / D 11%, D / D 4%, Tibetan I / I 16%, I / D 10%, D / D 7%, Mongolian I / I 14%, I / D 12% , D / D 6%, plain Han I / I 5%, I / D 3%, D / D 26%. Genotype frequency plateau three groups showed little difference in these three groups respectively compared with the plain difference between the groups was significant (P amp;lt;0.001). In addition, in Wistar rat HIF gene sequence 490bp sites have ACE gene expression. Conclusion In this study, further study of high altitude hypoxic adaptation of genetic mechanisms provide a basis for molecular biology. [Keywords:] Tibetan; Mongolian; Han; gene polymorphism; hypoxia Study of gene polymorphism and hypoxia related gene on residents livin at high altitude and plain area [Abstract] Objective This paper researched angiotensin converting enzyme (ACE) gene polymorphism of Tibetan, Mongolian, Han Chinese residents living at high altitude and Han Chinese living at plain area.Methods Applying the technology of polymearse chain reaction (PCR) gene amplifi