High CO2 on the invasion of gastric cancer cells.docVIP

 PAGE \* MERGEFORMAT 10 High CO2 on the invasion of gastric cancer cells Study: Health and Xu Hui Ren Fengming Renwu Jun Ni [Abstract] Objective: To investigate the CO2 peritoneal metastasis of gastric cancer cells. Methods: Cultured human gastric cancer MKN45 high invasive cell lines, high pressure CO2 (25mmHg, and different duration (1,2,3 hours after the intervention, the application flow Cellular technology to detect the expression levels of MMP2 and MMP9. Results: the role of high pressure CO2, the gastric cancer cell lines MKN45 MMP2 and MMP9 protein expression change, compared with conventional cell culture, MMP2 and MMP9 expression was significantly (P lt;0.01. Conclusion: High CO2 environment on invasiveness of cancer cells a greater impact, so that gastric cancer cell invasion and metastasis has been enhanced. [Key Words] high-pressure CO2, cancer cells In recent years, laparoscopic radical treatment of advanced gastric cancer achieved good results [1], but on whether CO2 pneumoperitoneum can improve the invasiveness of cancer cells, increasing opportunities for peritoneal metastasis is still controversial. Therefore, we chose a high aggressive ability of gastric cancer cell lines MKN45, build high CO2 environment, high pressure CO2 vitro on the expression of metalloproteinases MMP2 and MMP9 and peritoneal metastasis of gastric cancer cells ability to influence a wide range of clinical laparoscopic radical gastrectomy carried out to provide a theoretical basis. 1 Materials and methods 1.1 Cell culture and continuous high-pressure CO2 treatment on the selection of cells with high invasion of human gastric cancer MKN45 cell line (purchased from Shanghai Institute of Digestive Disease, Shanghai Renji Hospital, cells containing 10% fetal calf serum in the culture medium PRMI 1640, home into 37 , 5% CO2 incubator in regular culture medium was changed 48 hours time. trypan blue Reagent gastric cancer cell activitygt; = 95%, logarithmic gro