High-dose methotrexate treatment of children with acute lymphoblastic leukemia serum concentration monitoring and analysis.docVIP

 PAGE \* MERGEFORMAT 4 High-dose methotrexate treatment of children with acute lymphoblastic leukemia serum concentration monitoring and analysis [Abstract] Objective: A high performance liquid chromatography (HPLC) Determination of children with methotrexate (MTX) plasma concentrations, and high-dose methotrexate treatment of children with acute lymphoblastic leukemia (ALL) blood drug concentration monitoring The results were analyzed. Methods: from April 2005 to March 2007 were treated 21 cases of ALL children received 85 cases of high-dose MTX treatment, MTX at a dose of 5 g / (m2 * time). 42 h after application with MTX MTX-CF rescue. In the application of high-dose MTX after 44 h, 72 h using high-performance liquid chromatography method for monitoring plasma concentrations of MTX. Results: 44 h, 72 h MTX plasma concentrations were (0.82 ± 0.95) μ mol / L, (0.091 ± 0.062) μ mol / L. Conclusion: MTX disposal of individual differences in the body that it could under the blood concentration results as the adjustment of dosage of folinic the main basis for CF. [Keywords:] methotrexate in acute lymphoblastic leukemia, plasma concentration [Abstract] Objective: To determine blood concentration of methotrexate in human plasma by HPLC and analyze result of blood concentration monitoring of acute lymphoblastic leukemia (ALL) treated with high  dose  methotrexate (HD  MTX). Methods: 21 ALL hospitalized from 2005 to 2007 were given the curative scheme of eighty  five cycles of HD  MTX (5 g/m2). To rescue with calicium folinate 42 h after the treated with HD  MTX. Blood concentration of MTX was monitorred by high performance liquid chromatography in 44 h, 72 h after the administration in each cycle. Results: Blood concentration of MTX in 44 h, 72 h after the administration were (0.82 ± 0.95) μ mol / L, (0.091 ± 0.062) μ mol / L, respectively. Conclusions: MTX has a large individual variation in disposition, the dosage of calicium folinate increase o