High-performance liquid chromatography of Solanum nigrum in solasodine.docVIP

 PAGE \* MERGEFORMAT 11 High-performance liquid chromatography of Solanum nigrum in solasodine Author: Luo Xi-zhen Chen Jianjun Yao Pengcheng [Abstract] Objective To establish the solasodine in Solanum nigrum high performance liquid chromatography (HPLC) detection method. Methods methanol acidic water ultrasonic extraction nigrum alkaloids in the alkaloid glycosides under reflux conditions for acid hydrolysis, the corresponding solasodine. Followed by high-performance liquid chromatography, chromatographic conditions: column for the Diamonsil C18 column (250 mm * 4.6 mm), acetonitrile -0.05% heptafluoro butyric acid (30:70) system as the mobile phase, flow rate of 1.0 ml / min The detection wavelength was 210 nm. The results of Solanum nigrum alkaloid obtained a good baseline separation; Solasodine linear range of 102 ~ 612 μ g * ml-1 (r = 0.999 9); plus sample recovery rate is over 96.0%, RSD of 3.35% (n = 6). Conclusion The method is simple and rapid, accurate and reliable and can be used as medicine and its preparations Solanum nigrum quality control methods. The method for the development of Solanum nigrum quality standards and further pharmacodynamic study provides a reliable platform for data and methodology. [Keywords:] black nightshade; solasodine; high performance liquid chromatography Abstract: ObjectiveTo develop a new quantitative method for determining solasodine in Solanum nigrum L. by high-performance liquid chromatography (HPLC). MethodsAfter sonic extracted with acidic methanol, the alkaloids were acid hydrolyzed with reflux, and then eluted with acetonitrile-0.05% heptafluorobutyric acid as mobile phase. The chromatography parameters were as follows: column: Diamonsil ODS analytical column; flow rate: 1.0 ml / min; detection wavelength: 210 nm. ResultsBaseline separation was obtained for the alkaloids. Good linear range was found in the concentration range of 102 ~ 612 μ g / ml with r = 0.999 9. The recoveries were no less than 96.0%, RSD