High-performance liquid chromatography of cholesterol in rat hippocampus.docVIP

 PAGE \* MERGEFORMAT 4 High-performance liquid chromatography of cholesterol in rat hippocampus Author: Tan to Xun, Sun Sheng-gang, Wang Xi-Lin, Li Xiao-wide, the south Mei, Zhang pairs of countries [Keywords:] cholesterol [Keywords:] high-performance liquid chromatography L; cholesterol; rat hippocampus; UV detector 0 Introduction Cholesterol, not only in atherosclerosis and other cardiovascular diseases, the occurrence and development is crucial, but also in the development and function of the central nervous system activity plays a key role in [1]. This information is a relatively simple high-performance liquid chromatography ( high performance liquid chromatography, HPLC) at different developmental stages were determined by cholesterol content in rat hippocampus. 1 Materials and methods 1.1 Materials Experimental Animal Center of Hubei Academy of Medical Sciences provide a healthy male Wistar neonatal rats (within 24 h of birth), rats (28 ~ 30 d, weight 40 ~ 60 g) and adult rats (90 ~ 100 d, body weight 200 ~ 250 g ) of the 10. acetonitrile, isopropyl alcohol, ethanol and so pure reagent for chromatography, the standard cholesterol (HPLC grade) for the United States Sigma products. VARIAN Prostar 210 high-performance liquid chromatography (VARIAN U.S. company), configure Prostar 210 high-pressure pump, VARIAN Prostar 500 column temperature box, manual injector. 1.2 Methods 1.2.1 Preparation of tissue samples will be decapitated rats were quickly killed at the quality of the hippocampus, said, according to 10 mL / g plus ethanol homogenized in ice ground into the stage, at 20 600 g, 4 ℃ centrifugation two times, each time 20 min, the supernatant with 0.45 μ m micropore membrane filtration, take filtrate subpackage, -70 ℃ to save under test. 1.2.2 Preparation of cholesterol standard solution and determination of standards of cholesterol stock solution (1 g / L): Weigh accurately 66.18 mg of cholesterol dissolved in 25 mL of anhydrous ethanol. The