Histopathologically the technical improvement of fracture remedies.docVIP

 PAGE \* MERGEFORMAT 6 Histopathologically the technical improvement of fracture remedies [Abstract] Objective?: Looking for histopathology remedy is easy to produce a new fracture. Methods: easy to produce cracks in the organization of paraffin sections with ammonia and re-applied. Results: The approach of these organizations, the slices flat, well-structured , staining not significantly different. Conclusion: The pathology of the fissures ammonia remedies have significant results. [Keywords:] paraffin section; fracture; ammonia; softening In paraffin sections, we will often mix different sizes of fixed dehydrated specimens, which will inevitably be uneven sample preparation: inadequate treatment or large organization, or small organizations to address the over, making some tissue brittle and hard. biopsy often shows a missing edge of the “shell-like” (see Figure 1) and “blinds like” crack. The reason is often associated with increased tissue fragility, if this wax again After the line frozen sections, such cracks are more obvious and serious. In order to remedy this problem wax block, we brothers units with technology exchange and explore their own practice and found that the proper use of ammonia can soften the tissue and reduce tissue brittle, reducing the fracture to occur, and more conducive to well-cut paraffin wax. are as follows. 1 materials, methods and results 1.1?? Material?? Select our hospital in 2008 to organize routine paraffin wax paraffin cracks appeared 358, including lymph nodes, spleen, liver, kidney, uterus, breast and other surgical specimens were taken at the same time with endoscopic , needle biopsy specimens. reagents: Pure ammonia (Shanghai Chemical Reagent Co Mitaka) 500 mL. 1.2?? Way?? prone to fracture of such organizations wax into cold water (temperature at 0 ~ 5 ) about 10 min. slicer wax block placed carefully to expose the entire organization crude repair section, and then fine-cut wax more than 10 sheets (