Hormone therapy in children with primary nephrotic syndrome before and after the change of immunological parameters and significance of.docVIP

 PAGE \* MERGEFORMAT 12 Hormone therapy in children with primary nephrotic syndrome before and after the change of immunological parameters and significance of [Abstract] Objective To investigate the primary nephrotic syndrome (PNS) before and after hormone therapy in children with peripheral blood lymphocyte subsets, cytokine IL-2, IL-4 and immunoglobulin changes. Methods in children with newly diagnosed PNS 46 cases, the use of flow cytometry in peripheral blood lymphocyte subsets [CD3 +, CD4 +, CD8 +, CD4 + / CD8 +, CD19 +, CD (16 +56) +]; radioimmunoassay of serum interleukin -2 (IL- 2), IL-4 levels and nephelometry of serum immunoglobulin (IgG, IgM, IgA) levels, 36 patients with steroid-sensitive than those in remission review indicators. 20 cases of healthy children as control group. Results PNS activity in children with CD3 +, CD4 +, CD4 + / CD8 +, CD (16 +56) + cells were lower than in remission and control group (P lt;0.01), CD19 + cells higher than the remission stage and the control group (P lt;0.01); CD8 + cells in remission and control group no significant difference (Pgt; 0.05); remission in children with only CD4 + cells compared with control group decreased significantly (P lt;0.01), the remaining number of lymphocyte subsets compared with the control group not statistically significant; activity of IL-2 were higher than those in remission and control groups decreased significantly (P lt;0.01), IL-4 level was significantly increased (P lt;0.01); mitigate the level of IL-4 Although significantly lower than active, but still higher than the normal control group; activity of IgG with remission and control group were lower (P lt;0.01), IgM, IgA did not change. Conclusion PNS cellular immune function in children with , and the imbalance between lymphocyte subsets, cell subsets can be used as indicator of renal disease activity; PNS activity in children with decreased expression of IL-2, IL-4 increased, there is Th1/Th2 imbalance; PNS patients a