HPLC Determination of diisopropylamine dichloroacetate content.docVIP

 PAGE \* MERGEFORMAT 13 HPLC Determination of diisopropylamine dichloroacetate content Keywords: high-performance liquid chromatography; diisopropylamine dichloroacetate; Determination [Abstract] Objective: To establish HPLC Determination of the content of diisopropylamine dichloroacetate. Methods: Zorbax SBC18 column (250 mm × 4.6 mm, 5 μm), with 1.0 mmol / L tetrabutylammonium bromide solution (pH adjusted to 7.2 with ammonia)-acetonitrile (72:28) as mobile phase The flow rate was 1.0 mL / min, detection wavelength was 207 nm. Results: The two isopropylamine dichloroacetate at 0.5 ~ 1.5 mg / mL concentration range a good linear relationship (r = 0.9999), day and inter-day precision were 0.80%, 1.05%, the average recovery was 99.1%, the sample The destruction of degradation products and diisopropylamine dichloroacetate good separation. Conclusion: The method is simple, specific and can be used to diisopropylamine dichloroacetate Determination. [Keywords:] high-performance liquid chromatography; diisopropylamine dichloroacetate; Determination Determination of diisopropylamine dichloroacetate with HPLC [Abstract] Objective: To establish an HPLC method to determine the contents of diisopropylamine dichloroacetate. Methods: Zorbax SBC18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was consisted of 1.0 mmol / L tetrabutylammonium bromide solution ( adjusted pH to 7.2 with ammonia water) acetonitrile (72:28). The flow rate was 1.0 mL / min. And detection wavelength was set at 207 nm. Results: The calibration curve of diisopropylamine dichloroacetate was linear in range of 0.5 ~ 1.5 mg / mL. The RSD of intraday and interday assays was 0.80% and 1.05%, respectively. Satisfactory separation between diisopropylamine dichloroacetate and its main degradation product was obtained. Conclusions: The method is convenient, selective and effective for determination of diisopropylamine dichloroacetate. [Keywords:] HPLC; Diisop