HPLC Determination of Huperzine A Huperzine A sustained release tablets content.docVIP

 PAGE \* MERGEFORMAT 8 HPLC Determination of Huperzine A Huperzine A sustained release tablets content [Abstract] Objective To determine the huperzine A hydrophilic matrix sustained release tablets of the content of Huperzine A. METHODS HPLC, Kromasil C18 column, methanol -0.02% triethanolamine solution (volume ratio 60:40) as the mobile phase, detection wavelength was 309 nm, flow rate 1.0 mL min-1, the column temperature was 30 , and the injection volume 20 L. Results of Huperzine A in the 1.041 ~ 52.05 g mL-1 well within the range of linear relationship, r = 0.999 6. The average recovery was 99.37%, RSD = 0.40%. Conclusion The method is simple, accurate, specific, and can be used for the preparation of quality control. [Keywords:] huperzine A; hydrophilic matrix sustained-release tablets; high performance liquid chromatography of content; Abstract: Objective To establish a method for the determination of huperzine A in huperzine A hydrophilic matrix sustained release tablets.Methods HPLC was performed on a C18 column using mathanol 0.02% triethanolamine (60:40) as the mobile phase at a flow rate of 1 mL min-1. The detection wavelength was at 309nm. The column temperature was 30 and the sample size was 20 L.Results The linear range of huperzine A was 1.041 ~ 52.05 g mL-1 (r = 0.999 6), the average recovery was 99.37% (RSD = 0.40%). Conclusions The method is simple, accurate, special and can be used for the quality control of huperzine A hydrophilic matrix sustained release tablets. Keywords:: huperzine A; hydrophilic matrix sustained release tablets; HPLC; content determination Huperzine A from Huperzia Huperzia serrata plants alkaloids isolated one, as potent reversible cholinesterase inhibitors. Animal experiments indicate that Huperzine A can enhance the mouse learning and memory and so on [1]. clinically proven, its myasthenia gravis and senile amnesia, dementia has the exact effect [2], the treatment of Alzheimer’s disease mechanism m