HPLC Determination of sulfonamide piperidine of bilirubin in the ice Yellow Tablets.docVIP

 PAGE \* MERGEFORMAT 8 HPLC Determination of sulfonamide piperidine of bilirubin in the ice Yellow Tablets [Abstract] Objective To establish a high-performance liquid chromatography sulfonamide anchor ice Huang Tablets bilirubin level approach. Methods HPLC method was measured column for Dikma C18 (250 mm * 4.6 mm, 5 μ m); mobile phase: In order to chloroform - methanol -2% diethylamine aqueous solution (80:19:1), detection wavelength: 450 nm, flow rate: 1 mL / min, column temperature: 45 ℃ . The results of bilirubin, under certain chromatographic conditions can be completely separated, in the 0.62 ~ 1.85? Zi g / ml concentration range of linear relationship was good, the linear regression equation Y = 5 165 741X-61 229, γ = 0.999 9, plus sample recovery rate was 99.2%; RSD of 1.1% (n = 9). Conclusion The determination sulfonamide anchor ice yellow tablets of bilirubin, accurate and reliable method was convenient. [Keywords:] sulfonamide anchor ice yellow tablet; bilirubin; HPLC method; Determination Sulfur yellow piece of ice in recent piperidine clinical compound commonly used in a class of antimicrobial drugs, the current methodology contained in its mouth oxazole sulfamethoxazole, trimethoprim made a determination of the requirements [1], while one of the artificial bezoar content of control have not been asked. The main ingredient of artificial bezoar for bilirubin, so we use the content of external standard method of its determination for the drugs to provide a good basis for quality control. An experimental material 1.1 Instrument LC-10ATVP HPLC instrument (Shimadzu), SPD-10AVP UV detector (Shimadzu, Japan), chromatography workstation (Zhejiang University, N3000), AB135-S Electronic Analytical Balance (Mettler), AEU-210 E Analytical Balance (Xiang Yi), UV-2401 UV spectrophotometer (Shimadzu, Japan). 1.2 reagent Sulfonamide anchor ice pornographic films; Shanxi Jinhua Pharmaceutical Co., Ltd. (batch number2,003,050,320,030,801); bilirub