HPLC determination of VC Yinqiao Tablets phillyrin content.docVIP

 PAGE \* MERGEFORMAT 12 HPLC determination of VC Yinqiao Tablets phillyrin content 【Abstract】 Objective To establish a VC Yinqiao Tablets phillyrin method for the determination. Methods using high performance liquid chromatography using C18 column (4.0mm × 250mm, 5μm), acetonitrile - water (25:75) as mobile phase, detection wavelength 277nm. The results of the linear range of phillyrin 0.4 ~ 3.0μg; phillyrin The average recovery was 99.28%, RSD = 2.81% (n = 5). CONCLUSION The samples were measured using high sensitivity, good repeatability, can effectively control the quality of VC Yinqiao tablets. 【Key Words】 VC Yinqiao tablet; phillyrin; HPLC; assay An instrument and reagent Instrument: Waters 600E liquid chromatograph; Waters 600 pump, Waters2997 photodiode array detector; Millennium32 chromatography workstation. FA1004 Electronic Analytical Balance (Shanghai Ryohei Instrument Co., Ltd.), KQ3200 ultrasonic cleaner (China Kunshan Ultrasonic Instrument Co., Ltd.). Phillyrin (for HPLC use, China Pharmaceutical and Biological Products Institution, Lot :110821-200406). Reagent and sample: acetonitrile for HPLC pure, the other reagents were analytically pure, water re-distilled water, VC Yinqiao tablets (homemade). 2 Determination 2.1 Chromatographic conditions [1] column Symmetryshield (Rp18 4.6mm × 250mm); acetonitrile - water (25:75) as mobile phase; detection wavelength 277nm; flow rate 1ml/min; column temperature was 23 ℃. 2.2 Preparation of reference substance solutions that take precise amount of phillyrin reference substance, add methanol is made with 0.2mg per 1ml of solution, shake, that is, too. 2.3 Preparation of samples for test solution precision Weigh VC Yinqiao tablets after the removal of fine powder sugar-coated Institute 3.500g, home Gusset conical bottle, precision adding methanol 45ml, said the fixed weight, soaking overnight, ultrasound treatment