HPLC determination of the active ingredient content of the brain Dean granules.docVIP

 PAGE \* MERGEFORMAT 6 HPLC determination of the active ingredient content of the brain De’an granules [Abstract] Objective: To determine the content of the active ingredient particles of brain Tokuyasu method. Methods: The high-performance liquid chromatography (HPLC) De-particles in the brain content of Pueraria lobata were determined. Results: puerarin 0.081 ~ 0.41 μ g good linear relationship between the average recovery was 100.05%, RSD = 1.22% (n = 5). Conclusion: This method is sensitive, good reproducibility and is suitable for particles in the brain De’an Determination of Pueraria lobata. [Keywords:] Brain De-particle high-performance liquid chromatography of puerarin Brain De-particles from 37, Chuanxiong, safflower, and other traditional Chinese medicine composed of Pueraria. With the blood circulation, dredge the meridians, Xingnao Kaiqiao effect. De-particles in order to control the quality of the brain to ensure that clinical drug effects, this experiment established the HPLC determination of puerarin in cerebral De-particle approach. The methodology study confirmed the high specificity of the method, the recovery and precision is good, with satisfactory results. An experimental material Waters600 high-performance liquid chromatography; Waters 2487 UV-visible wavelength detector  ; Breeze chromatography workstation; one hundred thousandth micro-analytical balance; puerarin (purchased from Chinese medicine and biological products); methanol for chromatography pure; other reagents were AR; Brain De-particles made by the Court. 2 Methods and Results 2.1 Chromatographic conditions [1  2] Column: EclipseXDB  C18 (4.6 mm * 250 mm, 5 μ g); mobile phase: methanol - water (25:75); flow rate: 1.0 mL / min; column temperature was 25 ℃ ; detection Wavelength: 245 nm; injection volume was 10 μ g. 2.2 Preparation of reference substance solution obtained precision that puerarin reference substance 5 mg, buy 25 mL Liang Ping, add methanol to the sc