HPLC determination of αpurslane extract the content of linolenic acid and linoleic acid.docVIP

 PAGE \* MERGEFORMAT 14 HPLC determination of αpurslane extract the content of linolenic acid and linoleic acid Authors: Xin mass, Hou Yin-Huan, Li Min, Lu Jin-Cai Chang-Quan Ling [Abstract] Objective: Using High Performance Liquid Chromatography Determination of Portulaca oleracea L. extract α  linolenic acid and linoleic acid content, in order to extract the quality control of purslane to provide a quick and easy way. Methods: According to the following chromatographic conditions were analyzed. Column: Shim  pack CLC  ODS column (250 mm * 4.6 mm, 5 μ m); guard column: DIKMA EasyGuard C18 (10 mm * 4.6 mm); mobile phase: acetonitrile-methanol   0.5% phosphoric acid solution (60 ︰ 22 ︰ 18); column temperature: 26 ℃ ; detection wavelength: 210 nm; flow rate: 1.1 ml / min; injection volume: 25 μ l. Results: α  linolenic acid regression equation A = 2.915 8 * 107 C 12 250.9, r = 0.999 9, the linear range of 0.016 2 ~ 0.194 4 mg / ml, the average recovery was 100.5%. Linoleic acid regression equation is A = 1.366 4 * 107 C-9 759.39, r = 0.999 9, the linear range of 0.016 9 ~ 0.203 0 mg / ml, the average recovery was 100.8%. Conclusion: The high-performance liquid chromatography method is simple, accurate, rapid and can be used as purslane extract α  linolenic acid and linoleic acid in quantitative analysis method. [Keywords:] purslane; herbal extracts; α  linolenic acid; linoleic acid; chromatography, high performance liquid Objective: To determine α  linolenic acid and linoleic acid in extract of Portulaca oleracea L. by high performance liquid chromatography (HPLC). Methods: The determination was done with a Shim  pack CLC  ODS (250 mm * 4.6 mm, 5 μ m) and a DIKMA Easyguard C18 (10 mm * 4.6 mm). Elution was employed with the mobile phase of methanol  acetonitrile  0.5 % phosphonic acid (60 ︰ 22 ︰ 18) at flow rate of 1.1 ml / min. Column temperature was 26 ℃ . Detection wavelength was 210 nm. Injection volume was 25 μ l. Re