HPLC in the determination of plasma homocysteine levels of.docVIP

 PAGE \* MERGEFORMAT 8 HPLC in the determination of plasma homocysteine levels of Author: Wu Jian Ling, Shen Jian-Xin Xue-Mei Bai [Abstract] Objective: To investigate the high-performance liquid chromatography (HPLC) in the determination of plasma homocysteine levels in the application. Methods: The plasma homocysteine with o-phthalaldehyde (OPA) derivatives by Hypersil AA  ODS column (2.1mm * 200mm, 5μ m), using 0.02 mol * L-1NaAc: acetonitrile: tetrahydrofuran = 56:43.8: 0.2 The mobile phase elution, column temperature 40 ℃ , detection wavelength 330nm, flow rate 1.3mL * min-1. Results: The linear range of 0.48 ~ 31.25μ g * mL-1, the regression equation is A = 27.167C 38.765, r = 0.9962; The average recovery was 98.61%, RSD = 1.84 (n = 6), day and day relative standard deviations were less than 5%. Conclusion: The method is accurate, simple, fast and suitable for most laboratories. [Keywords:] Homocysteine; high-performance liquid chromatography [ABSTRACT] Objective: To set up the method for determining the content of Homocysteinein Human Plasma with HPLC. Methods: Column was Hypersil AA  ODS (2.1mm * 200mm, 5μ m), using 0.02mol * L  1Sodium acetate  Acetonitrile  Tetrahydrofuran (56:43.8:0.2) Balanced Solution as mobile phase and detective wavelength at 330nm, the fiowing speed was 1.3ml * min-1, and Column temperature was 40 ℃ . Results: The calibration curve was linear in the range of 0.48 ~ 31.25 μ g * mL-1 and, The linear correlation equation was A = 27.167 38.765 (r = 0.9962) The average recovery rate was 98.61%, and RSD was Between 1.84% (n = 6). The standard relative difference within a day and between two days was lower than 5%. Conclusion: The method is simple, accurate, and fast, which is suitable for the most lab test. [KEY WORDS] Homocysteinein; HPLC Homocysteine (Homocysteine, Hcy) is a sulfur-containing amino acid, methionine cycle is the S  adenosyl-homocysteine hydrolase base hydrolysis product. Studies have shown that