HPLC method for the detection ambush itraconazole Content of legislation.docVIP

 PAGE \* MERGEFORMAT 9 HPLC method for the detection ambush itraconazole Content of legislation [Abstract] Objective To establish human serum samples ambush legislation itraconazole concentration detection methods. Method according to Bartlett C18 column for the column (5μ m, 4.60mm * 200mm); mobile phase water: acetonitrile: acetic acid: triethylamine = 64:36:0.1:0.05; flow rate: 1.2ml/min; detection wavelength: 258nm . Results Voriconazole in the concentration range of 0.14 ~ 13.96μ g/ml showed good linear relationship, r = 0.998. Conclusion The establishment of the HPLC method can be used in serum samples voriconazole concentration detection. [Keywords:] Voriconazole HPLC Determination of serum samples [Abstract] Objective To establish a method to determinate the voriconazole content in human serum samples.Methods Eliter C18 column (200mm * 4.6mm, 5μ m) was used with the mobile phase of water-acetonitrile-acetic acid-triethylamine (64:36: 0.1:0.05). The flow rate was 1.2ml.min-1and the wave-length was 258nm. Results The calibration curve was linear in the range of 0.14-13.96μ g/ml (r = 0.998). Conclusion The established HPLC method can be used in the determination of voriconazole content in human serum. [Keywords:] voriconazole; serum sample; HPLC; determination Voriconazole (voriconazole, VRC) is a triazole antifungal drug, its mechanism is to inhibit fungal cytochrome p450-dependent 14α -sterol demethylase, while this product for the fungal cytochrome p450 enzymes than for various mammalian The cytochrome p450 enzyme system more selective. Voriconazole concentration in the range of 0.03 ~ 64μ g/ml almost all of the experiments within the fungi, including pathogenic yeasts and filamentous genus and two-budded both the role of bacteria isolates. For some conditions, such as pathogenic fungi Aspergillus, Cryptococcus, and Candida are included fluconazole-resistant Candida krusei have antibacterial activity. This study was designed to establish an ambus