HPLC method for simultaneous determination of 3 kinds of pills Zhuanggu joint content of active ingredient.docVIP

 PAGE \* MERGEFORMAT 10 HPLC method for simultaneous determination of 3 kinds of pills Zhuanggu joint content of active ingredient Of: Yang Jing, Tan Pei, Pan Guixiang, an era, Guo Jinming [Abstract] Objective To establish the simultaneous determination of icariin in Zhuanggu joint pills, psoralen and isopsoralen HPLC method for content. Methods Zorbax SB C18 (4.6 mm 150 mm, 5 m) column, mobile phase: acetonitrile 0.05% formic acid (gradient elution, detection wavelength: 0 8 min to 270 nm, 8 15 min to 246 nm, flow rate: 1.0 mL / min, column temperature was 35 . Results of icariin , psoralen, isopsoralen at 0.0223 ~ 0.713 g (r = 0.9997,0.0272 ~ 0.869 g (r = 0.9998,0.0213 ~ 0.681 g (r = 0.9998 and peak area within the linear relationship The average recoveries were 101.1% (RSD = 3.27%), 97.66% (RSD = 2.46%), 99.65% (RSD = 2.13%). Conclusion The method is simple, reproducible, and can be used as strong bone joint pills quality control methods. [Keywords:] Zhuanggu joint pills, icariin, psoralen, isopsoralen; gradient elution Abstract: Objective To establish an HPLC-VWD method for simultaneous quantification of icariin, psoralen and isopsoralen in Zhuangguguanjie pills by using switching wavelength. Methods The separation was performed on a Zorbax SB-C18 (4.6 mm 150 mm, 5 m) column using the gradient elution which consisted of acetonitrile and 0.05% formic acid aqueous solution at the flow rate of 1.0 mL / min. The detection wavelength was at 270 nm in the first 8 min, while changed to 246 nm at 8-15 min. Column temperature was fixed at 35 . Results For icariin, psoralen and isopsoralen, the linear ranges were 0.0223-0.713 g (r = 0.9997), 0.0272-0.869 g (r = 0.9998) and 0.0213-0.681 g (r = 0.9998), respectively, and their average recoveries were 101.1% (RSD = 3.27%), 97.66% (RSD = 2.46%) and 99.65% (RSD = 2.13%), respectively. Conclusion The method could be used for the quality control of Zhuangguguanjie pills, which was available with a good repro