HPLC method for the determination of silver Po detoxification caffeic acid content in the film.docVIP

 PAGE \* MERGEFORMAT 8 HPLC method for the determination of silver Po detoxification caffeic acid content in the film Of: Limei Rong ON TRADITIONAL Huang Bin, Lin Jie [Abstract] Objective To establish a silver film Po detoxification method for determination of caffeic acid. Method using high performance liquid chromatography, the choice of C18 column (4.6 mm × 250 mm, 5 μm), methanol -0.1% phosphoric acid (26: 74) as the mobile phase, flow rate 1.0 mL / min, column temperature 30 ℃, the detection wavelength was 323 nm. the results of caffeic acid in the sample volume in the range of 0.778 ~ 2.72 μg peak area was a good linear relationship (r = 0.999 9 ), the average recovery was 98.80%, RSD = 0.56%. Conclusion The method was simple, accurate, and can effectively control the quality of silver films Po detoxification. [Keywords:] Po detoxification of silver film, caffeic acid, HPLC, determination Abstract: Objective To establish the method for determination of caffeic acid in Yinpujiedu Tablets by HPLC. Methods Stationary phase was Lichrospher-C18 (4.6 mm × 250 mm, 5 μm), mobile phase was methanol-0.1% phosphoric (26:74), detection wavelength was 323 nm, flow rate was 1.0 mL / min and column temperature was 30 ℃. Results The calibration curve were linear in the range of 0.778 ~ 2.72 μg for caffeic acid (r = 0.999 9). The average recovery rate was 98.80 %, RSD was 0.56%. Conclusion This method is simple and convenient for the operation. It is very accurate and suitable for the quality control of Yinpujiedu Tablets. Keywords:: Yinpujiedu Tablets, caffeic acid, HPLC, content determination Po detoxification of silver film is Lonicera confusa, dandelion, violet, Prunella vulgaris, wild chrysanthemum plus accessories sucrose, talc, magnesium stearate made of sugar coated tablets, with heat detoxification for the treatment of acute pharyngitis and so on. is not yet available in the literature related to the preparation of caffeic acid content. In ord