HPV18 E7 antigen HLA-DRB1 0301 restricted Th epitope prediction and identification of.docVIP

 PAGE \* MERGEFORMAT 7 HPV18 E7 antigen HLA-DRB1 0301 restricted Th epitope prediction and identification of [Abstract] Objective: Preliminary identification of human papillomavirus type 18 E7 antigen HLA-DRB1 * 0301 restricted secondary T lymphocytes (T-Helper Cell) epitopes. Methods: SYFPEITHI software predicts that HPV18 E7 antigen, HLA-DRB1 * 0301 restricted Th epitope, the candidate epitopes were named P1, P2, P3, to solid phase peptide synthesis synthesized and purified using HPLC and mass spectrometry (MS ) Identification. By density gradient separation of HLA-DRB1 * 0301-positive healthy human peripheral blood mononuclear cells (PBMC), synthetic peptides to stimulate PBMC, using 5 - bromo-deoxyuridine (Brdu) Detection of lymphocyte proliferation activity by flow cytometry cell phenotype. Results: The peptide P1 (HPV18 E780-94) after in vitro stimulated PBMC can effectively induce CD4 T lymphocyte proliferation. Conclusion: P1 (HPV18 E780-94) may HPV18 E7 antigen HLA-DRB1 * 0301 restricted Th cell epitope. [Keywords:] Human papillomavirus E7 antigen epitopes Th lymphocytes Abstract: Objective: To identify HLA-DRB1 * 0301restricted Th epitope derived from human papillomavirus 18 E7 antigen. Methods: The primary structure of human papillomavirus 18 E7 antigen was analyzed using T cell epitope predictive algorithms SYFPEITHI and three epitope candidates (P1, P2 and P3) were obtained. The candidate epitopes were synthesized with solid phase strategies, purified with reverse phase HPLC and identified with mass spectrometry. Peptide-specific Th cells were induced from the peripheral blood mononuclear cells (PBMC) of HLA-DRB1 * 0301 positive healthy donors by stimulating candidate epitopes. Proliferative activity was analyzed with Brdu cell proliferation assay. Cell phenotype was analyzed by FACS. Results: Peptide P1 (HPV18 E780-94) could induce proliferative activity in vitro specifically and effectively. Conclusion: Peptide P1 (HPV18 E780 -94) can be the H