Human sperm mannose receptor on hamster oocyte ultrastructure.docVIP

 PAGE \* MERGEFORMAT 11 Human sperm mannose receptor on hamster oocyte ultrastructure Author: Jiang Shu, Lin Dian Liang, Chen columns, Jiang Yiping [Abstract] Objective To study the human sperm mannose receptor (MR) in the sperm-egg fusion in the role. Ways to zona hamster eggs by different concentrations of purified human sperm after the MR effect, using transmission electron microscopy Oocytes ultrastructure changes. The role of the results of MR oocytes, the oocyte cortical granules within the (CG) reduced and accompanied by the phenomenon of CG exocytosis, while oocytes reduce the number of surface microvilli. Conclusion MR acting on the oocyte cell surface, may trigger the CG exocytosis, and to promote oocyte microvilli reduced, suggesting that MR in mediating the sperm and oocyte recognition and fusion play a role. [Keywords:] mannose; sperm; sperm egg interaction; membrane fusion; oocyte The last century, 90 years studies have found that the existence of human sperm head, the complementarity of mannose molecules, and prove that such elements are directly related with the fertilization process. Because such molecules have a mannose receptor-like effect, so called mannose receptor (mannose receptor, MR). Gabriele, etc. I and preliminary studies suggest that MR may be of human sperm recognition and sperm-egg membrane fusion mediated by the time when the role of [1  3]. I purified by affinity chromatography of MR to replace the sperm directly on to the zona pellucida of the golden hamster oocytes, using transmission electron microscopy observation of changes in the oocyte to understand the MR in the role of sperm-egg membrane fusion. 1 Materials and methods 1.1 Materials 1.1.1 masturbation semen specimen collection method collected for the health and normal fertile sperm semen, routine semen analysis of the indicators in line with WHO standards [4]. 1.1.2 Animal 6 ~ 8-week-old Syrian golden hamster (Mesocricetus auratus, Experimental Animal Cen