Huang TF on primary cultured rat hepatocytes injury.docVIP

 PAGE \* MERGEFORMAT 4 Huang TF on primary cultured rat hepatocytes injury Author: Su-Ting, YANG He-mei, Zhou Xiaohui [Keywords:] Wong, TF;,, liver cells;,, primary culture;,, protection;,, mechanism Abstract: Objective To study the Huang TF (SSTF) on primary cultured rat liver cells from oxidative injury and its mechanism. Methods trypsin digestion, separation of rat liver cells in primary culture, using H2O2 and CCl4-induced liver cell injury in vitro at the same time to different concentrations of SSTF to protect the liver cells to detect the culture medium alanine aminotransferase (ALT) the activity of liver cells of malondialdehyde (MDA) content and glutathione peroxidase (GSHpx) activity, MTT determination of liver cell proliferation activity. Results Compared with model group, SSTF significantly inhibited H2O2 and CCl4 induced liver cell culture medium in the ALT activity increased significantly higher survival rate of liver cells, but also significantly restored H2O2 induced liver MDA content increased and the activity of GSHpx reduced significantly. Conclusion SSTF on cultured rat liver cell injury has obvious protective effect and its mechanism may be related to lipid oxidation effects. Keywords:: Yellow SSTF; liver cells; primary culture; protection; mechanism Study on the Protective Effect and Possible Mechanism of Scutellaria baicalensis Stem-leaf Total Flavonoid on Primary Cultured Rat Hepatocytes Injured by H2O2 or CCl4 Abstract: ObjectiveTo study the protective effect and possible mechanism of Scutellaria baicalensis stem-leaf total flavonoid (SSTF) on primary rat hepatocyes injured by H2O2 or CCl4.MethodsPrimary hepatocytes were isolated, cultured by trypsin digestion and induced by H2O2 or CCl4.Various concentrations of SSTF were added to the primary cultured hepatocytes. The contents of malondialdehyde (MDA) and activity of glutathion peroxidase (GSH-px) in hepatocytes and the activity of ALT in cultural supernatant were determined by g