HPLC Determination of Five yellow paste of gallic acid.docVIP

 PAGE \* MERGEFORMAT 6 HPLC Determination of Five yellow paste of gallic acid [Abstract] Objective To establish a five yellow paste of gallic acid determination. Methods using high performance liquid chromatography, using methanol -0.1% phosphoric acid as the mobile phase gradient elution; a flow rate of 1.0 mL / min; detection wavelength was 270 nm . Results of gallic acid in the linear range of 0.189 2 ~ 1.892 μg (r = 0.999 9), the average recovery was 99.2%, RSD = 1.22%. Conclusion The method is efficient, rapid, sensitive, five yellow paste can be used as an effective quality control methods. [Keywords:] five yellow paste; gallic acid; High Performance Liquid Chromatography Determination of content; Abstract: Objective To set up a method for determining the content of gallic acid in Wuhuang Gao. Methods HPLC separation was performed by gradient eluting with methanol-0.1% phosphoric acid at the flow rate of 1.0mL/min. The detection wavelength was 270 nm . Results The linear range of gallic acid was 0.189 2 ~ 1.892 μg, r = 0.999 9. The average recovery rate was 99.2%, RSD = 1.22%. Conclusion The method is highly effective, quick and sensitive, and suitable for quality control of Wuhuang Ointments. Keywords:: Wuhuang Ointments; gallic acid; HPLC; content determination Five yellow paste is gall, skullcap, borneol compound composed of 3 herbs preparation, clearing and detoxification, swelling and pain, OPHTHALMOLOGY, dehumidifying effect of convergence [1], clinically for the treatment of sties and boils, a paronychia. gallnut is arguably the main drug, gallic acid as its main active ingredient. for the effective control of product quality, the author of five yellow paste the establishment of gallic acid by high performance liquid chromatography (HPLC) method. An instrument and reagent Shimadzu HPLC, binary gradient pump, SPD-M20A detector, SIL-20AC autosampler, CTO-20A oven, LC-Solution chemistry workstation. Gallic acid standard (batch