HPLC Determination of double yellow Tongbi particles ephedrine and pseudoephedrine content.docVIP

 PAGE \* MERGEFORMAT 11 HPLC Determination of double yellow Tongbi particles ephedrine and pseudoephedrine content [Abstract] Objective To establish a compound preparation of double yellow Tongbi particles ephedrine, pseudoephedrine, method for the determination. Method column for Akasil C18 (4.6 mm * 250 mm, 5 μ m); mobile phase of 0.02 mol / L potassium dihydrogen phosphate (containing 0.3% diethylamine, phosphoric acid adjusted pH = 3)  acetonitrile (volume ratio 96:4 ); detection wavelength 210 nm; a flow rate of 1.0 mL / min; column temperature 40 ℃ ; injection volume of 10 μ L. The results of ephedrine in the 25.2 ~ 504 μ g / mL showed good linear relationship within the average recovery was 101.23%; pseudo-ephedrine in the 17.6 ~ 352 μ g / mL showed good linear relationship within the average recovery was 99.25%. Conclusion This method is simple feasible, reproducible, and can be used to double yellow Tongbi granules active ingredient ephedrine, pseudoephedrine, by HPLC. [Keywords:] double yellow Tongbi particle; ephedrine; pseudoephedrine; HPLC . Abstract: Objective To determine the contents of ephedrine and pseudoephedrine in Shuanghuangtongbi granula. Methods HPLC was used to determine the content of ephedrine and pseudoephedrine in Shuanghuangtongbi granula. Samples were analyzed on an Akasil C18 column (4.6 mm * 250 mm, 5 μ m) . The mobile phase consisted of 0.02 mol / L potassium dihydrogen phosphate (0.3% diethylamine, phosphoric acid accommodate pH3)-acetonitrile (96:4). The column temperature was 40 ℃ and flow rare 1.0 ml / min at a detection wavelength of 210 nm. Results A good linearity was obtained over 25.2-504 μ g / mL of ephedrine and 17.6-352 μ g / mL of pseudoephedrine for plasma with a good correlation coefficient. The average recoveries of ephedrine and pseudoephedrine were 101.23% and 99.25%, respectively. Conclusion This method is simpleand quick. Which could be used for the quality control of Shuanghuangtongbi granula. Keywor