HPLC determination of double flowers on the ear drops the content of chlorogenic acid.docVIP

 PAGE \* MERGEFORMAT 7 HPLC determination of double flowers on the ear drops the content of chlorogenic acid [Abstract]: Objective To establish a double-take ear drops in the determination of chlorogenic acid. Methods HPLC, Kromasil C18 column (5 μm, 4.6 mm × 250 mm), mobile phase of acetonitrile -0.4% phosphoric acid solution (15:85), detection wavelength was 328 nm, flow rate: 1.0 mL / min, column temperature: room temperature. The average recovery was 99.8% and relative standard deviation was 1.51% (n = 5). Conclusion This established high performance liquid chromatography of chlorogenic acid in 0.102 4 ~ 0.614 4 μg good linear relationship. [Keywords:] Double spend eardrops chlorogenic acid by high performance liquid chromatography Abstract: Objective To establish the method of content determination of chlorogenic acid in Shuanghua eardrop. Methods High performance liquid chromatography (HPLC) was adopted with Kromasil C18 column (5 μm, 4.6 mm × 250 mm) and acetonitrile-0.4% phosph acid solution ( 10:85) as the mobile phase. The detective wavelength was 328 nm, the flow rate was 1.0 mL / min, column temperature was room temperature. Results Average recovery was 99.8%, relative standard error was 1.51% (n = 5). Conclusion The linearity relationship of chlorogenic acid was good at the range of 0.102 4 ~ 0.061 44 μg. Keywords:: Shuanghua eardrop, chlorogenic acid, HPLC Double flowers ear drops in Gansu Province Chinese Medicine Hospital of the Chinese herbal compound developed by the honeysuckle, forsythia composed of 4 herbs, with swelling and pain, detoxify the abscess, grabbing wet rot Qu, myogenic effect, clinical treatment for otitis media. The complexity of prescription components, in order to control drug quality, we used high performance liquid chromatography (HPLC) method in which contents of chlorogenic acid was determined [1], satisfactory results can be used as quantitative control of the preparation indicators. An instrument and